Bypassing Translation Initiation

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Translation Initiation System

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lacZ translation initiation mutations.

Sixteen single point mutations near the beginning of the lacZ gene have been isolated and their effect on lacZ expression has been measured. Five mutations were obtained that alter a potential stem-and-loop structure in the messenger RNA that masks the initiation codons. Formation of this stem-and-loop is a result of transcription of DNA sequences introduced during the cloning of the lac regula...

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ژورنال

عنوان ژورنال: Structure

سال: 2007

ISSN: 0969-2126

DOI: 10.1016/j.str.2006.12.002